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bceA [2019-09-02 12:52:59]
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bceA [2019-09-02 12:52:59]

primary bacitracin resistance determinant, ABC transporter (ATP-binding protein) for the export of bacitracin, plectasin, mersacidin and actagardine
Locus
BSU_30380
Isoelectric point
9.29
Molecular weight
28.08 kDa
Protein length
253 aa Sequence Blast
Gene length
762 bp Sequence Blast
Function
export of toxic peptides
Product
bacitracin ABC transporter (ATP-binding protein)
Essential
no
Synonyms
ytsC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
  • Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,111,327 3,112,088

    Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin PubMed

    • The protein

    Protein family

  • ABC transporter family (according to UniProt)

    • Paralogous protein(s)

  • YknY, YtrE, YxdL, PsdA, YvrO

    • Effectors of protein activity

  • the activity of the BceA-BceB ABC transporter is inhibited by heptaprenyl pyrophosphate which accumulates in a ytpB mutant PubMed

    • Structure

  • 1L2T (MJ0796 from Methanocaldococcus jannaschii , 38% identity) PubMed

    • Localization

  • membrane associated (via BceB) PubMed

    • Expression and Regulation

    Operons

    Genes
    bceA-bceB
    Description
    PubMed

    Regulatory mechanism

  • BceR: activation, PubMed, in BceR regulon

    • Regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin (BceR) PubMed
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    Biological materials

    Mutant

  • MGNA-A167 (ytsC::erm), available at the NBRP B. subtilis, Japan
  • BKE30380 (bceA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
  • BKK30380 (bceA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT

    • Labs working on this gene/protein

  • Foglino M. PubMed

    • References

    Reviews

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    Original publications

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